The announcement comes after rival Microsoft announced major investments in Malaysia, Thailand and Indonesia last week.
Amazon has announced plans to invest $9 billion in Singapore to expand its cloud infrastructure.
The announcement at the Amazon Web Services (AWS) ASEAN Summit on Tuesday came after the Seattle-based technology giant announced multibillion-dollar investments in cloud services in Malaysia and Thailand last year.
Priscilla Chung, AWS Country Manager We will double our investment in structures.”
Amazon said the investment will support more than 12,000 jobs in Singapore businesses every year and add $23.7 billion worth of value to Singapore's gross domestic product (GDP).
Amazon's announcement is the latest in a series of moves by US-based tech giants to expand their footprint in Southeast Asia.
Last week, Microsoft announced multibillion-dollar investments in artificial intelligence (AI) and cloud services in Malaysia, Thailand, and Indonesia.
Nvidia CEO Jensen Huang visited Malaysia, Vietnam, and Singapore in December, and Apple CEO Tim Cook visited Singapore, Vietnam, and Indonesia last month.
On Monday, Malaysian Trade and Investment Minister Tunku Zafrul Aziz said Google would announce plans to invest in the country “in the near future,” state news agency Bernama reported.
Global consulting firm Kearney estimates that AI could contribute nearly $1 trillion to Southeast Asia's GDP by 2030.
Amazon was the world's largest cloud infrastructure provider through AWS during the first three months of this year, according to Synergy Research Group.
The tech giant reported last week that operating profit tripled to $15.3 billion, driven primarily by performance in its advertising and cloud businesses.